4.5. Cell Viability MTT Assay

Cells were plated at a density of 40,000 cells per well in a 96-well plate. Cells were incubated with various concentrations of atRAL for 24 h. Cell viability was determined using MTT (M6494; Thermo Fisher Scientific). Following dosing, treatment media was replaced with 100 µL fresh media. MTT was prepared at a concentration of 5 mg/mL in PBS, and 10 µL was added to each well. Cells were incubated for 4 h at 37 °C. All but 25 µL media was removed, and MTT was solubilised with 50 µL DMSO. Absorbance was read at 540 nm on a Safire2 plate reader (TECAN, Männdorf, Switzerland).