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4.2. Cell Viability Assay
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Pemetrexed-treated cells seeded in 96-well plates were exposed to different concentrations of EGF-TKI (gefitinib or erlotinib) in triplicate for 72 h. We performed cell viability assays according to the sulforhodamine B (Sigma-Aldrich) method [47]. IC50 was determined and indicated 50% growth inhibition compared with the control group.
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