3.10. In Vitro Drug Release Analysis

A drug release study was conducted at both acidic (pH 1.2) and basic media (pH 7.4) to analyze the percent release of commercial product of DS (Cataflam) and pH-dependent release of DS from CS/CBP-co-PAA hydrogels network. Samples were placed in 900 mL solutions of pH 1.2 and 7.4 in USP dissolution apparatus-II at 37 ± 0.5 °C and 50 rpm. The dissolution medium was collected at a specific interval of time and the same quantity of fresh medium was added back to keep the sink condition constant. The collected samples were then analyzed on UV–vis-spectrophotometer (UV-1601 Shimadzu) at 260 nm [83,84]. Kinetic modeling such as the Korsmeyer–Peppas model was carried out for all the developed formulations of CS/CBP-co-PAA hydrogels to understand the drug release mechanism from the hydrogels network [85].