4.1.2. The Smart Dentine Grinder Protocol

The protocol consists of two solutions used after the milling process. Particles of Ø 0.25 to 1 mm were obtained. The chemical solutions are (I) a dentine cleaner (0.5 mL Sodium Hydroxide with Ethanol) and (II) Dulbecco’s Phosphate Buffered solution. After the milling process, the particles were stored in a sterile container (mixing dish), and the dentine cleanser was used in order to cover the entire particles for 5 min. After this first phase, the cleanser was removed, and the buffered solutions were added to clean the residues. This step, according to the manufacturer’s instructions, was applied two times (Figure 5).

Workflow for the Smart Grinder protocol: (1) removal of tartar and periodontal ligament Figure (2). milling process, (3) particles ready to be chemically treated, (4) particles in the mixing dish, and (5) particles after the chemical process.