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2.5. MAO-A Activity Assay

GP Giovanni Pallio
AD Angela D’Ascola
LC Luigi Cardia
FM Federica Mannino
AB Alessandra Bitto
LM Letteria Minutoli
GP Giacomo Picciolo
VS Violetta Squadrito
NI Natasha Irrera
FS Francesco Squadrito
DA Domenica Altavilla
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MAO-A activity was evaluated by a two-step bioluminescent assay methodology, as previously described [32], using a MAO-GLOTM assay kit from Promega (Madison, WI, USA). The produced amount of signal (light intensity) was measured using a microplate luminometer (Multilabel Counter Victor3TM, PerkinElmer Life Sciences). Human recombinant MAO-A enzyme was used as a positive control. The results are given as relative light units after the background was subtracted.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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