2.11. In Vivo Microdialysis and GABA and Glutamate Determination

In vivo microdialysis in the cerebellum was performed as in [28]. Rats were anesthetized with isoflurane and a microdialysis guide was implanted in the cerebellum (AP −10.2, ML −1.6, and DV −1.2). After 48 h, a microdialysis probe was implanted in the freely moving rat. Probes were perfused (3 μL/min) with artificial cerebrospinal fluid (in mM): NaCl, 145; KCl, 3.0; CaCl2, 2.26; buffered at pH 7.4 with 2 mM phosphate. After a 2 h stabilization period, samples were collected every 30 min and stored at −80 °C until analysis of GABA and glutamate levels.

Extracellular basal concentration of GABA and glutamate was measured by HPLC-MS as in [28] in five consecutive microdialysis samples.