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Antimicrobial susceptibility testing using broth microdilution method

AA Abdelazeem M. Algammal
HH Hany R. Hashem
AA Amenah S. Al-otaibi
KA Khyreyah J. Alfifi
EE Esraa M. El-dawody
EM Eman Mahrous
HH Helal F. Hetta
AE Ali W. El-Kholy
HR Hazem Ramadan
RE Reham M. El-Tarabili
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The detection of minimal inhibitory concentrations (MICs) of the tested antimicrobial agents was carried out using the broth microdilution method according to the procedures of CLSI [33]. The test was performed using Middlebrook 7H10 broth (Oxoid, UK). The MIC for each antibiotic for each tested isolate was the mean of two repeated tests. Mycobacterium avium ATCC 700898 was used as a control strain. The MIC breakpoints of the tested antimicrobial agents were expressed as sensitive, intermediate, and resistant as described by the CLSI guidelines. The tested isolates were classified into Multidrug-resistant (MDR: the resistance to at least isoniazid and rifampicin), Extensively drug-resistant (XDR: resistant to isoniazid and rifampicin as well as to fluoroquinolones, and at least one of the second-line drugs such as kanamycin, amikacin, and capreomycin), and Pan-drug resistant (PDR: resistant to all antimicrobial agents listed) according to Prasanna and Niranjan [34].

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