ROS and lipid peroxidation levels were measured as previously described [23, 24]. Cells were seeded in six-well plates 24 h prior to RT and incubated for another 24 h; 4 μM CM-H2DCFDA (ThermoFisher, C6827, USA) for the total ROS measurements or 5 μM BODIPY 581/591 C11 dye (Invitrogen, D3861, USA) for measurements of the lipid peroxidation levels was added per well, along with fresh medium. After incubation (30 min), cells were washed with PBS, trypsinized to a cell suspension with Trypsin–EDTA solution (Biosharp, Shanghai, China), and analyzed via flow cytometry (FACSCalibur; BD Biosciences, New Jersey, USA).
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