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2.5. Immunohistochemistry (IHC)

SW Shirong Wang
QC Qiang Cao
XC Xin Cui
JJ Jia Jing
FL Fenfen Li
HS Huidong Shi
BX Bingzhong Xue
HS Hang Shi
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IHC staining of UCP1 was conducted as we described [34,35]. Briefly, adipose tissues were fixed in 10% neutral formalin, embedded in paraffin and sectioned, followed by processing for hematoxylin and eosin (H&E) staining or immuno-staining with a UCP1 antibody (1:150, Abcam, ab10983) as we previously described [34,35]. Histology images were captured using a Nikon Eclipse E800 Microscope.

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