2.13. Bleomycin-Induced Skin Fibrosis Model

Skin fibrosis was induced by intradermal injection of bleomycin (BLM) sulfate (100 μL, 500 µg/mL in vehicle) into a single location on the shaved back skin of C57BL/6 mice once daily for 3 weeks. A total of 40 mice were equally randomized into five groups: (1) NaCl group, where mice were injected with vehicle (100 μL sterile saline plus 1% DMSO) intradermally; (2) BLM group, where mice were treated with 100 μL BLM sulfate intradermally; (3–5) treatment groups, where mice were treated with 100 μL BLM plus pinocembrin (20, 40, 80 μM) intradermally.