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This assay helps to check the effect of Cr-SPs on bacterial colony formation ability. Overnight culture of bacterium was diluted to 106 CFU/mL and treated with Cr-SPs in concentration ranging from 0.5 to 8 mg/mL for 24 h. After incubation the culture was appropriately diluted, and 50 μL were plated on GM-3 agar plates and incubated for 24 h at 37 °C. After 24 h the colonies were counted manually, and the data is used for viability analysis [40].
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