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TUNEL assay

ZC Zuxiang Chen
YG Yanzhi Ge
LZ Li Zhou
TL Ting Li
BY Bo Yan
JC Junjie Chen
JH Jiefeng Huang
WD Wenxi Du
SL Shuaijie Lv
PT Peijian Tong
LS Letian Shan
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TUNEL (terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labelling) assay was employed to observe the apoptosis of chondrocytes, following the manufacturer’s instructions. Briefly, the tissues were permeabilized with the proteinase K solution at 37 °C for 20 min, followed by incubation with the TUNEL detection liquid at 37 °C for 1 h. After washing with PBS for three times, DAPI was added to stain nuclei, and the tissues were examined via a fluorescence microscope (Carl Zeiss). The rate of apoptotic cells was quantified in three randomly selected fields of view by using three slides from each sample.

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