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Pain behavior evaluations

ZC Zuxiang Chen
YG Yanzhi Ge
LZ Li Zhou
TL Ting Li
BY Bo Yan
JC Junjie Chen
JH Jiefeng Huang
WD Wenxi Du
SL Shuaijie Lv
PT Peijian Tong
LS Letian Shan
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The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured by Electronic tenderness tester (Huaibei Zhenghua, China) and Plantar Test apparatus (Ugo Basile, Italy), respectively. All rats were individually placed in elevated plastic cages with wire mesh bases and given 30 min for acclimatization to the testing environment. The room temperature and humidity were kept stable throughout the experimental period. For the test of MWT, the sensing probe of electronic tenderness tester was pressed perpendicularly against the midplantar surface of the left and right hind paws of each rat more than three times. For the test of TWL, a focused beam of radiant heat (up to 35 °C) was irradiated to the plantar surface of the hind paws more than three times and held for maximal 20 s. A positive response for each test was regarded as the sharp withdrawal of the paw and paw licking.

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