2.3.2. Kautsky Curve Fluorescence Induction Decay (KC)

The measuring and super pulses were the same as in the light curve analysis (LC). The actinic light was set to 300 µmol photons m⁻² s⁻¹. After the initial saturating super pulse, actinic light was switched on and lasted for 1 min. Within this time period, five additional super pulses were applied, the first one after 7 s and the following in 12 s intervals. The subsequent dark period lasted 88 s, during which three super pulses were applied, the first one after 11 s and the following in 26 s intervals. The fluorescence parameters F₀, F_M, F_M′, F(t), F₀″, and F_M″ (Figure S1) allowed calculating a number of coefficients (Table S2), among which non-photochemical quenching (NPQ), energy-dependent non-photochemical quenching coefficient (q_E), and photoinhibitory photochemical quenching coefficient (q_I) informed about non-photochemical quenching (NPQ) and photochemical quenching (PQ) together with the photochemical quenching coefficients q_P and q_L about photochemical quenching (for detailed explanations, see Table S2).