Flow cytometric analysis of cell cycle with propidium iodide DNA staining

MZ Ming Zhang
JW Jian Wang
KZ Kaixiang Zhang
GL Guozhen Lu
YL Yuming Liu
KR Keke Ren
WW Wenting Wang
DX Dazhuan Xin
LX Lingli Xu
HM Honghui Mao
JX Junlin Xing
XG Xingchun Gao
WJ Weilin Jin
KB Kalen Berry
KM Katsuhiko Mikoshiba
SW Shengxi Wu
QL Q. Richard Lu
XZ Xianghui Zhao
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PI staining for flow cytometry was performed according to the user manual of DNA Content Quantitation Assay (Cell Cycle) from Solarbio (#CA1510). Briefly, OPCs from control or Tet1 cKO mice were harvested, washed in PBS, and fixed in cold 70% ethanol for 30 min at 4 °C. After washing twice in PBS, cells were treated with RNase and then stained with PI. With guava easyCyte6HT (Millipore), the forward scatter (FS) and side scatter (SS) were measured to identify single cells. For analysis, ModFit LT 5.0 software was used to make the PI histogram plot. Experiments were replicated three times.

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