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In SPECT/CT imaging, 99mTc-DCN (~500 μCi for each mouse) was injected into KM mice (n=5) via the tail vein without anesthesia. After 1 h post injection, the mice were anesthetized by inhaling the isoflurane (1.5%)-oxygen mixture, and placed prone on the scanning bed for SPECT/CT imaging by nanoScan SPECT/CT with large FOV (12 cm X 10 cm). CT scanning was performed following each SPECT acquisition. SPECT and CT images were reconstructed using the MEDISO software and then merged and analyzed using the InVivoScope software (Bioscan).
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