Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Preparation of Transforming DNA (tDNA)

LZ Li Zhang
LH Li Huang
MH Mi Huang
MW Mengying Wang
DZ Dekang Zhu
MW Mingshu Wang
RJ Renyong Jia
SC Shun Chen
XZ Xinxin Zhao
QY Qiao Yang
YW Ying Wu
SZ Shaqiu Zhang
JH Juan Huang
XO Xumin Ou
SM Sai Mao
QG Qun Gao
BT Bin Tian
AC Anchun Cheng
ML Mafeng Liu
request Request a Protocol
ask Ask a question
Favorite

Transforming DNA (tDNA) for natural transformation experiments was amplified from the strain R. anatipestifer ATCC 11845 ΔRA0C_1551 using the primers RA0C_1551 upP1 and RA0C_1551 downP2. The tDNA contains the upstream of RA0C_1551, the Erm antibiotic resistance cassette, and the downstream of RA0C_1551. The strain R. anatipestifer ATCC11845ΔRA0C_1551 was constructed by transformation-mediated recombination in our previous study (Liu et al., 2017b). The fragments were purified using the TianGEN Extract II Kit (TIANGEN, Beijing, China). The concentration of the fragments was measured by a NanoDrop 2000 spectrophotometer.

Copyright and License information:  ©2021 Zhang, Huang, Huang, Wang, Zhu, Wang, Jia, Chen, Zhao, Yang, Wu, Zhang, Huang, Ou, Mao, Gao, Tian, Cheng and Liu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A