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Gait was captured using the catwalk gait analysis system 7.1 (Noldus). Mice voluntarily ran across a glass platform, and a camera mounted below captured the footsteps. Up to six runs were recorded for each mouse at each timepoint and the three straightest, most consistent runs are selected for analysis. Image analysis of the gait pattern was carried out using the Catwalk analysis software (Noldus). The gait parameters analysed for each model correlated with those previously identified as being informative for tracking with disease progression23,25.
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