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Monocyte isolation:

AY Anjana Yadav
AK Andrew V. Kossenkov
LS Louise C. Showe
SR Sarah J. Ratcliffe
GC Grace H. Choi
LM Luis J. Montaner
PT Pablo Tebas
PS Pamela A. Shaw
RC Ronald G. Collman
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Monocytes were purified for gene expression analysis at weeks 0, 6, 12, and 18 (washout). Blood was collected in EDTA tubes and peripheral blood mononuclear cells (PBMC) separated by Ficoll-gradient centrifugation. CD14+ monocytes were then isolated by negative selection with antibody-conjugated magnetic beads according to the manufacturer's instructions (Miltenyi Biotech), suspended in RNAlater (Ambion), and frozen at −80°C until RNA extraction. FACS analysis was done on each isolation and based on CD14+ staining, mean monocyte purity was 93% (range: 92%-94%).

Copyright and License information: Pathogens and Immunity ©2021
This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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