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Alkaline Phosphatase Assay
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BMSCs were seeded into six-well plates at a density of 2 × 103 cells/well, and treated with different concentrations of PT, as described in 2.4. Cells were treated with PT for 3 and 7 days, and ALP activity assays were completed using ALP kits according to the manufacturer's instructions (A059-2-2, Nanjing Jiancheng, China). Absorbance was measured at 520 nm using a multi-well spectrophotometer (BioTek, Synergy H4).
Copyright and License information: ©2021 Li, Cui, Lin, Wang, Chen, Zhu, Yang and Zhang.
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