PT was twice extracted by decoction in distilled water (2 h each time), and then concentrated via rotary evaporation to a final concentration of 0.1 g/ml. After cooling the extracts to room temperature, PT was stored at −20°C under dry, airproof, and non-polluted conditions until further use. Qualitative amino acid analyses of the PT extracts were performed using a Hitachi L-8900 automatic amino acid analyzer (Tokyo, Japan).
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