Combination Effect of Chemotherapy and A009 on Cancer Cell Lines

AA Adriana Albini
MF Marco M. G. Festa
NR Nadja Ring
DB Denisa Baci
MR Michael Rehman
GF Giovanna Finzi
FS Fausto Sessa
SZ Serena Zacchigna
AB Antonino Bruno
DN Douglas M. Noonan
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To investigate whether the A009 extract could synergize with chemotherapy, the prostate cancer DU-145 cell line or the colorectal cancer HT-29 cell line were treated with Cis-Pt 100 µM or 5-FU 100 μM, respectively, alone or in combination with A009 L3 or L4 extracts, for 24–72 h. Detection of cell viability was determined by MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) assay, on 3,000 cardiomyocytes/well, seeded into a 96 well plate.

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