To investigate whether the A009 extract could synergize with chemotherapy, the prostate cancer DU-145 cell line or the colorectal cancer HT-29 cell line were treated with Cis-Pt 100 µM or 5-FU 100 μM, respectively, alone or in combination with A009 L3 or L4 extracts, for 24–72 h. Detection of cell viability was determined by MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) assay, on 3,000 cardiomyocytes/well, seeded into a 96 well plate.
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