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Matrigel (50 µL) was coated on 96-well plate allowed to solidify at 37ºC for 1 h. HUVECs were seeded on Matrigel cushion at the density of 2 x 104 cells/well. Then the cells were treated with 2 µg/mL of BmMP-1 or BaMP-1, 100 ng/mL of BmLAAO or BaLAAO, 10 ng/mL of bFGF and PBS (Control) and incubated at 37°C with 5% CO2 for 6 h. Kinetics of tube formation at different time points was observed and counted.
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