For CLSM, Skov3 cells (5 × 105) were seeded in 6-well plates and cultured for 12 h. After treated with Ir-NPs (10 μM) at 37 °C for 2, 4, and 6 h, the cells were fixed with 4% (W/V) paraformaldehyde for 15 min. Then, the nuclei of the cells were stained using DAPI (2 μg/mL, blue) for 5 min. At last, images were collected with CLSM. Emission was collected at 440 ± 20 nm for DAPI and 590 ± 200 nm for Ir-NPs upon excitation at 405 nm. For flow cytometry, after treated with Ir-NPs (10 μM) for 2, 4, and 6 h, the cells were collected and resuspended with PBS for detection.
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