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Protoplasts were transformed using previously reported methods (Peyyala and Farman, 2006). The hygromycin selection plates were surveyed daily to identify transformants (colonies with smooth, hazy borders), and colonies were carefully monitored for sudden cessation of growth (indicative of telomere crisis). Hyphal tips were then carefully excised, being extremely careful not to include hyphae from neighboring, untransformed individuals. A number of normally growing, “ectopic” transformants were picked as controls.
Copyright and License information: ©2021 Rahnama, Wang, Dostart, Novikova, Yackzan, Yackzan, Bruss, Baker, Jacob, Zhang, Lamb, Stewart, Heist, Hoover, Calie, Chen, Liu and Farman.
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