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Immunoblotting

TF Tiffany R. Frey
IA Ibukun A. Akinyemi
EB Eric M. Burton
SB Sumita Bhaduri-McIntosh
MM Michael T. McIntosh
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Akata, HH514-16, LCL, or BCBL-1 cells were harvested after the respective time points, lysed, and immunoblots were performed as previously described (Koganti et al., 2014) using primary antibodies of interest. Mouse anti-β-actin (AC-15; Sigma) or rabbit anti-GAPDH (2118S; Cell Signaling) were used as cellular loading controls. All immunoblotting was performed using 10% SDS-PAGE gels. Densitometry values were calculated by comparing protein of interest to β-actin or GAPDH controls of the same sample before normalizing to the control condition using Image Studio software.

Copyright and License information:  ©2021 Frey, Akinyemi, Burton, Bhaduri-McIntosh and McIntosh.
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