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The recombinant pBI121 vectors (pBI121-GhLUX1 and pBI121-GhELF3) constructed in the gene cloning step were transferred into the Agrobacterium tumefaciens strain GV3101 and were transformed into Arabidopsis via the floral dip method (Clough and Bent, 1998). The positive plants were selected on 1/2MS medium containing kanamycin (50 mg/L), and further confirmed via PCR and qRT-PCR.
Copyright and License information: ©2021 Hao, Wu, Chen, Wang, Ma, Wei and Yu.
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