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Alkaline Phosphatase (AP) Activity Staining
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Frozen uterine sections were fixed with 4% paraformaldehyde in PBS for 10 min at 4°C and washed with 1 × PBS three times for 5 min. The uterine sections were then washed in PBS, followed by incubation at room temperature in an AP substrate solution containing 5-bromo-4-chloro- 3-indolyl phosphate (Amresco, United Sates) and nitro blue tetrazolium (Amresco, United Sates).
Copyright and License information: ©2021 Zhu, Wu, Chen, Kang, Pan, Liu, Li, Yan, Liu, Huang, Yang and Su.
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