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Dynamic light scattering (DLS) experiments were carried out on Zetasizer Nano-ZS instrument (Malvern Pananalytical Ltd., UK). The purified protein was used at a concentration of one mg/ml in different buffers with and without the reducing agents. The measurements were carried out in low volume disposable sizing cuvette (ZEN0112 of Malvern, UK) at 20 °C. The samples were equilibrated at 20 °C for 30 s followed by three measurements, with every measurement having 15 runs of 10 s each. The scattered light was measured at a backscattering angle of 173°.
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