The survey was conducted by experienced research assistants who were trained in the study protocols. All samples taken were marked with a participant identification number and recorded in a register. Two certified microscopists, who were blinded to the RDT results, carried out the microscopy for malaria parasites. A third microscopist was consulted in the case of any divergence greater than or equal to 50%. The HemoCue system and microscopes were calibrated and verified prior to use in the study. The RDT kits were approved by the Food and Drugs Authority (FDA), as recommended by the Ghana National Malaria Control Programme (NMCP). The questionnaire was completed and verified by the data collection team for accuracy, consistency and completeness.
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