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Sampling and identification of predator and competitor macroinvertebrates

HO Hudson Onen
RO Robinson Odong
MC Moses Chemurot
FT Frédéric Tripet
JK Jonathan K. Kayondo
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Each randomly picked quadrat for An. gambiae s.l. larvae and other aquatic insects was sampled by dipping standard 350 ml dippers (BioQuip Products, Inc., CA, USA) four times per quadrat. Samples collected from each quadrat in the morning and afternoon were combined and sieved using a stainless-steel mesh strainer (Innovative Lab Instruments, New Delhi, India). Sorting of invertebrates was done in the field, and samples were preserved in 5 ml falcon tubes (Fisher Scientific Ltd., UK) containing 80% ethanol. They were transported to the Entomology laboratory at the Uganda Virus Research Institute (UVRI) in Entebbe and identified by morphological characteristics to family level with the aid of a microscope (Opto-Edu Co., Ltd., Beijing, China) at ×40 eyepiece magnification. The identification of macroinvertebrates was carried out with the use of guides by Gerber and Gabriel [42] and Gill [43], while guides by Hopkins [44] and Rozeboom and Stone [45] were used for the identification of mosquito larvae. The identified individuals were counted.

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