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Briefly, macrophages (2×105 cells/well) were incubated in a 12-well plate with 1.6 mL BFM. The cells were divided into four treatment groups: 100 ng/µL CGS21680, 100 ng/µL ZM241385, 13.16 µL DMSO, and 100 ng/µL CGS21680+10 μM GW4869. After incubation for 48 h, the cell suspension was harvested. VECs were resuspended in the harvested cells and added to a Matrigel-coated 12-well plate (105 cells/well). After incubation for 12 h, vascular tube formation was observed. Details of the analysis methods are presented in Supplementary Material.
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