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Cell lines and reagents

XZ Xuexiu Zhang
JY Jianning Yao
HS Haoling Shi
BG Bing Gao
HZ Haining Zhou
YZ Yanzhen Zhang
DZ Dongyao Zhao
SG Shilin Gao
CW Chunfeng Wang
LZ Lianfeng Zhang
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Human normal colorectal mucosal cell line (FHC) and 4 human CRC cell lines (SW620, LOVO, SW480, SW116) were used for the study, and all of them were available from ATCC Company (Manassas, VA). DMEM (Invitrogen, Carlsbad, CA) supplemented with 10% FBS and 1% antibiotics were applied for cell culture under 5% CO2 at 37 °C. For treating SW480 and LOVO cells, 2 μg/ml of ActD and DMSO (Mock) were procured from Sigma-Aldrich (St. Louis, MO), while 3 U/μg of RNase R was bought from Epicentre Technologies (Madison, WI). LiCl (5 mg), an activator of the Wnt signaling pathway, was purchased from Med Chem Express (Monmouth Junction, NJ).

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