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Cellular uptake of welding fume particles

SM Sarah McCarrick
VR Valentin Romanovski
ZW Zheng Wei
EW Elin M. Westin
KP Kjell-Arne Persson
KT Klara Trydell
RW Richard Wagner
IO Inger Odnevall
YH Yolanda S. Hedberg
HK Hanna L. Karlsson
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To study the uptake of welding fume particles in HBEC cells, 50,000 cells/well were seeded in 24-well plates. Cells were exposed to welding fume particles at 25 µg/mL, or released fractions generated from 25 µg/mL welding fume particles for 24 h. Following exposure, media were removed and 0.5 mL of concentrated aqua regia (25 vol% HCl, 75 vol% HNO3) was added directly in the well for 1 h. The suspension was further transferred to a 1.5 mL tube (Safe Lock Tubes, Eppendorf AG) and the well was rinsed with an additional 0.5 mL of concentrated aqua regia added to the same tube and incubated in a ventilated hood at room temperature for 5 days. Prior to analysis, a volume of 200 µL of sample was added to 4.8 mL of ultrapure water in a 15 mL tube (Corning, #CLS430791).

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