2.10. Survival Analysis of Mesothelial Cells

The MTT assay is an instrument for detecting the number of living cells. Approximately 5 × 10³ mesothelial cells were inoculated on several 96-well plates for 12 h, and then, mesothelial cells were treated with distinct concentrations of H₂O₂ (1000 μM, 500 μM, 250 μM, 125 μM and 62.5 μM). Approximately 24 h later, the cells were soaked in 20 μL MTT at a concentration of 5 mg/mL for 4 h, and the MTT crystals were soluble in DMSO. Cell viability was measured by a microplate reader (Thermo Fisher Scientific, Waltham, USA).