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Concise Method
2.6. Protein Concentration Assay in BALF
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We lavaged the lungs thrice using 0.5 ml of cold saline (overall volume 1.5 ml) through tracheostomy with a tracheal cannula to obtain BALF. After centrifugation, a small portion of the supernatant was assayed for total protein concentration with the BCA (bicinchoninic acid) method approach.
Copyright and License information: ©2021 Yue Tang et al.
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