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Backcrossing of sni1-1 (Col-0) Mutant to Ler Background for GBS.
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To obtain sni1-1 mutant in Ler background, the sni1-1 (Col) allele was crossed to Ler-0. F1 plants obtained were backcrossed six times to Ler as recurrent female parent, and each generation was checked to carry sni1-1 mutation by genotyping (SI Appendix, Table S30). The genotype of the BC6 line was verified using 39 previously described Col/Ler indel markers (37).
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