Total RNA extraction and qRT-PCR

CL Chang Liu
HL Haifeng Li
XL Xiaojing Li
XZ Xuejing Zhao
XZ Xia Zhang
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Following the manufacture’s procedure, total RNA was extracted from cells. First, cDNA was synthesized with SYBR®PrimeScrip™RT-PCR kit (Takara) at 37 °C for 30 min. The expression of MANCR was examined by qRT-PCR using SYBR Premix ExTaq (Takara) reagent. The primers used were provided in Additional file 1: Table 1. The 2−ΔΔCt was used for quantitation.

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