The specific quantity of ingredients used in PCR amplification was as follows; DNA (2 µl), F-Primer (1 µl), R-Primer (1 µl), dNTP (10mM) mixture (0.3 µl), Taq(5U/µl)0.2 µl, 10×Buffer mg2+ 2 µl and ddH2O (13.5 µl). The specific PCR amplification procedure was as follows: 95 °C pre-denaturation for 5 min; 95 °C denaturation for 30 s, 56 °C annealing for 30 s, 72 °C extension for 1 min, 34 cycles; 72 °C extension for 7 min, 4 °C storage for 1 min. The PCR products were separated on 3 % agarose gel electrophoresis and photographed under a Gel Documentation System (Bio-Rad Gel Doc 2000).
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