xCGE-LIF analysis

LC Léa Chuzel
SF Samantha L. Fossa
MB Madison L. Boisvert
SC Samanta Cajic
RH René Hennig
MG Mehul B. Ganatra
UR Udo Reichl
ER Erdmann Rapp
CT Christopher H. Taron
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xCGE-LIF-based glycoanalysis was performed as described [68]. Samples were run on glyXboxCE systems (glyXera GmbH, Magdeburg, Germany) based on modified Applied Biosystems 3130 or 3130xl genetic analyzers using a 50 cm capillary array filled with POP-7™ polymer. For injection, samples were prepared using 1–3 µL of HILIC-SPE purified APTS-labelled samples, adding 0.5 µL of GeneScan™ 500 LIZ™ size standard diluted 1:50 in Hi-Di™ formamide, 0.5 µL of xCGE-LIF migration time normalization standard 2nd NormMiX and Hi-Di™ formamide up to a total sample volume of 12 µL. Samples were electrokinetically injected and separation was at 30 °C at 15 kV. Data were analyzed using the glycoanalysis software glyXtoolCE (glyXera).

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