The normality of data distribution was tested using the Wilk–Shapiro normality test. Normally distributed data, presented as mean ± standard deviation (SD), were compared by unpaired Student’s t-test for two groups comparisons and one-way analysis of variance (ANOVA) and followed up with a post hoc Bonfferoni test for multiple comparisons. Categorical variables, presented as n (%), were analyzed by the chi-square test or Fisher’s exact test, as appropriate. All p < 0.05 from two-sided tests were accepted as statistically significant. All statistical analyses were performed using SPSS 23.0 software (SPSS Inc., Chicago, Illinois) and GraphPad Prism 7 (GraphPad Software, La Jolla, California).
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