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Sample collection

EP Edoardo Pozio
MC Mario Celli
AL Alessandra Ludovisi
MI Maria Interisano
MA Marco Amati
MG Maria Angeles Gómez-Morales
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In 2017, as part of health checks, blood samples were collected from the jugular vein from breeding sows (n = 63, Large White × Duroc) and one boar (Duroc) 30 days before and 86 days after the outdoor access and tested for anti-Trichinella antibodies. As shown in Fig. 2, 40 days after fertilization, sows had outdoor access in a fenced area with a boar (for the control of estrus in sows) for a 2-month period. Then, sows were again kept indoors for 8 days before moving to the farrowing room for 4 weeks. As control group, 70 fattening and 14 breeding animals without outdoor access in the study period were also tested. Sera were obtained after centrifugation of clotted blood and kept at − 20 °C until further analysis.

Timeline scheme of sows and boar outdoor access and blood sampling. Red bars: period during which the sows and the boar had access to the walking area: blue bar: sows in the delivery room

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