2.16. Cell immunofluorescence

The HAVSMCs were cultivated on cell slices in 12-well plates for staining. After treatment, cells were fixed by 4% paraformaldehyde for 15 min, followed by permeabilized with 0.1% Triton X-100 for 5 min, and then 10% fetal bovine serum was used to block nonspecific binding sites. The cell slides were co-incubated VDAC (1:200) with Drp1 (1:50), LC3 (1:50), Parkin (1:50) at 4°C overnight. After incubating with secondary antibody for 1 h and staining with DAPI, the images were obtained by confocal laser microscope.