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BrdU Incorporation Assay (Proliferation Assay)
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BrdU incorporation was used to reflect the proliferation of cells after transfections. Briefly, 3000 cells were seeded into each well of a 96-well plate. At 48 h post-transfection, BrdU was added (10 µM), and cells were cultured for another 24 h. After that, cells were fixed and incubated with peroxidase-coupled anti-BrdU-antibody (Sigma-Aldrich) for 60 min. Following PBS washing, peroxidase substrate was used to incubate with the cells for 60 min. OD values (450 nm) were then measured to reflect cell proliferation.
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