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2.5. The spheroid-based sprouting angiogenesis model

YG Yaru Guo
FM Feng Mei
YH Ying Huang
SM Siqin Ma
YW Yan Wei
XZ Xuehui Zhang
MX Mingming Xu
YH Ying He
BH Boon Chin Heng
LC Lili Chen
XD Xuliang Deng
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For the in vitro sprouting angiogenesis assay, spheroids were generated overnight, after which they were embedded into gelatin methacryloyl (GelMA) gels (EFL, China). After light curing, culture medium was added. The spheroids were allowed to sprout for a few hours. Then, in vitro sprouting was quantitated digitally by measuring the number of extensions and the length of the sprouts (calculated as cellular invasion distance) that had grown out of each spheroid using the Image Pro Plus software, and analyzing 8–10 spheroids per experimental group.

Copyright and License information:  ©2021 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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