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Matrix Mineralization Analysis

ZS Zhe Shi
QZ Qiang Zhong
YC Yuhang Chen
JG Jian Gao
XP Xin Pan
QL Qiang Lian
RC Rong Chen
PW Pinkai Wang
JW Jian Wang
ZS Zhanjun Shi
HC Hao Cheng
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The effects of HAP and/or SN nanoparticles on mineralized extracellular matrix production in two-dimensional (2D) and 3D conditions were determined by Alizarin Red staining. After 14- and 28-days of culture, 2% (w/v) Alizarin Red S (pH = 4.2) was added to the cells and samples at room temperature for 10 min. For quantification, 10% (v/v) acetic acid was applied for a 30-min acidification of the stained samples. Subsequently, the acetic acid was neutralized with 10% ammonium hydroxide, and the resultant supernatant was measured using a microplate reader at a wavelength of 405 nm.

Copyright and License information:  ©2021 Shi et al.
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