Gel digestion with proteinase K (for SAXS)

MP Molly Phillips
AW Alauna C. Wheeler
MR Matthew J. Robinson
VL Valerie Leppert
MJ Manping Jia
MR Marco Rolandi
LH Linda S. Hirst
CA Chris T. Amemiya
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Equal volumes of AoL gel and 1X PBS were combined in a tube and vortexed vigorously. Proteinase K was added to the gel sample to a final concentration of 0.1 μg/μL and incubated at 50°C for one hour. Sample was then dialyzed with 3.5-KDa dialysis tubing in 2 L of water for 5 hours.

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