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L6-GLUT4myc myotubes and 3T3-L1-GLUT4myc adipocytes were incubated in the wells of a 24-well plate containing serum-free medium for 3 h at 37 °C and then incubated in glucose-free medium for 30 min at 37 °C. The cells were treated with the indicated concentrations of DS20060511 or insulin for 30 min at 37 °C, followed by the addition of 1 mM 2-deoxy-D-glucose (2-DG) and 0.3 μCi mL−1 [3H]-2-DG for 10 min. 2-DG uptake was measured with a liquid scintillation counter (Tri-Carb 2810TR, PerkinElmer, Waltham, MA) and normalized to the level in the vehicle-treated group.
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