Cell cultures

Human myoblasts (ID: hSkMC-AB1190) were immortalized as previously described [48]. Human myoblasts were cultured in Skeletal Muscle Cell Growth Medium (PromoCell, C-23060). Mouse 10T1/2 fibroblasts (ATCC, CCL-226) and C2C12 myoblasts (ATCC, CRL-1772) were maintained in 10% FBS with 1% penicillin/streptomycin (Gibco, 15140122) in DMEM (Dulbecco’s Modified Eagle’s Medium-high glucose, D5796). Primarily myoblasts were isolated by enzyme digestion following the protocol [80]. Human and mouse myoblast differentiation medium contains 2% horse serum supplemented in DMEM with 1% antibiotics penicillin/streptomycin. Cells were tested mycoplasma negative using Universal Mycoplasma Detection Kit (ATCC, 30-1012K).