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Polyethylene glycol (PEG) purification
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Precipitation buffer PEG 8000 solution was added to the unpurified 3D cuboid origami sample solution with an equal volume43. A thorough mix was then subjected to centrifugation at 16,000 g for 25 min at 25 °C. We removed the supernatant and dissolved the obtained pellet in 0.5 × TAE buffer supplemented with 35 mM MgCl2. Then the purified samples were directly used or incubated overnight at 25 °C.
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